Patterns of DNA-labeled and scFv-antibody-carrying lipid vesicles directed by material-specific immobilization of DNA and supported lipid bilayer formation on an Au/SiO2 template

被引：116

作者：

Svedhem, S

Pfeiffer, I

Larsson, C

Wingren, C

Borrebaeck, C

Höök, F ^{[1
]}

机构：

[1] Chalmers Univ Technol, Dept Appl Phys, S-41296 Gothenburg, Sweden

[2] Gothenburg Univ, S-41296 Gothenburg, Sweden

[3] Lund Univ, Dept Immunotechnol, S-22007 Lund, Sweden

来源：

CHEMBIOCHEM | 2003年 / 4卷 / 04期

关键词：

DNA; protein chips; supported lipid bilayers; surface patterning; vesicles;

D O I：

10.1002/cbic.200390055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spots before the eyes: A material-specific surface modification protocol was developed to allow surface-directed immobilization of DNA-labeled lipid vesicles. The potential of similarly immobilized vesicles to act as carriers for water-soluble or transmembrane proteins in, for example, protein-chip applications was demonstrated. The picture shows how fluorescent vesicles labeled with different DNA strands have been directed to one of two complementary-DNA-modified Au spots surrounded by an inert supported lipid bilayer on SiO2.

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页码：339 / 343

页数：5

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