A pre-tRNA carrying intron features typical of Archaea is spliced in yeast

被引：12

作者：

Di Segni, G ^{[1
]}

Borghese, L ^{[1
]}

Sebastiani, S ^{[1
]}

Tocchini-Valentini, GP ^{[1
]}

机构：

[1] CNR, Ist Biol Cellulare, I-00016 Monterotondo, Italy

来源：

RNA | 2005年 / 11卷 / 01期

关键词：

BHB; M; jannaschii; RAT1; tap1-1; tRNA splicing;

D O I：

10.1261/rna.7138805

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Archaeal pre-tRNAs are characterized by the presence of the bulge-helix-bulge (BHB) structure in the intron stem-and-loop region. A chimeric pre-tRNA was constructed bearing an intron of the archaeal type and the mature domain of the Saccharomyces cerevisiae suppressor SUP4 tRNA(Tyr). This pre-tRNA(ArchEuka) is correctly cleaved in several cell-free extracts and by purified splicing endonucleases. It is also cleaved and ligated in S. cerevisiae cells, providing efficient suppression of nonsense mutations in various genes.

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收藏

页码：70 / 76

页数：7

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