Sequence dependent rigidity of single stranded DNA

被引：253

作者：

Goddard, NL ^{[1
]}

Bonnet, G

Krichevsky, O

Libchaber, A

机构：

[1] Rockefeller Univ, Ctr Studies Phys & Biol, New York, NY 10021 USA

[2] Univ Strasbourg, F-67000 Strasbourg, France

来源：

PHYSICAL REVIEW LETTERS | 2000年 / 85卷 / 11期

关键词：

D O I：

10.1103/PhysRevLett.85.2400

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

Single stranded DNA (ssDNA) equilibrium dynamics are investigated using a fluorophore/quencher-labeled hairpin structure which thermally fluctuates between open and closed states. Temporal correlations of the fluorescence fluctuations are used to determine the energy barrier tu conformational change. We find that ssDNA distortion is purely entropic for poly(T) but requires an additional enthalpy of +0.5 kcal . mol(-1) base(-1) for poly(A), consistent with the disruption of base stacking. Such sequence dependent dynamics challenge the classical model of ssDNA as a completely flexible coil.

引用

页码：2400 / 2403

页数：4

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