Comparative analysis of FA, NL and sterol profiles in the Nephrops norvegicus (langoustine), Palinurus vulgaris (lobster) and Penaeus kerathurus (shrimp) muscle and cephalothorax showed that C16:0, C16:1 omega-7, C18:0, C18:1 omega-9, C20:4 omega-6, EPA and DHA were found to be their major FA. Highest EPA occurred in langoustine muscle TL, DHA in both shrimp tissues TL while C20:4 omega-6 in lobster muscle and cephalothorax TL. Muscle and cephalothorax NL consisted mainly of sterols (42.5-54.4% and 13.7-43.1%) and triacylglycerols (35.4-45.8% and 44.6-59.4%). Cholesterol was the major sterol (70.90-98.58% and 97.10-98.31% of total sterols in muscle and cephalothorax respectively) followed by avenasterol (0.18-20.32% and 0.07-0.70%) and beta-sitosterol (0.29-7.30% and 0.23-0.75%). Lower concentrations of brassicasterol, stigmasterol, Delta 7-stigmasterol, campesterol and campestanol were also found. The examined crustaceans muscle (edible part) was found to be a good omega-3 PUFA source for the consumers, while the cephalothorax (which is usually discarded) could be used effectively as a source for omega-3 PUFA production. (C) 2010 Elsevier Ltd. All rights reserved.