Estrogen-mediated up-regulation of the Ca-dependent constitutive nitric oxide synthase in the rat aorta and heart

被引：16

作者：

Morschl, E

Bretus, I

Nemcsik, J

László, F

Pávó, I

机构：

[1] Hungarian Acad Sci, Inst Expt Med, H-1083 Budapest, Hungary

[2] Univ Szeged, Fac Med, Dept Med 1, H-6701 Szeged, Hungary

[3] Lilly Area Med Ctr, A-1031 Vienna, Austria

来源：

LIFE SCIENCES | 2000年 / 68卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

nitric oxide; eNOS enzyme regulation; estrogen; cardiovascular protection; ischemic heart disease;

D O I：

10.1016/S0024-3205(00)00921-8

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The role of endogenous estrogens has been studied in the regulation of the Ca-dependent constitutive nitric oxide synthase (cNOS) enzyme activity in aortic and cardiac tissues of the rat. The activity of cNOS enzyme was measured by the citrulline assay in the abdominal aorta and in the left ventricle of the heart obtained from male, sham-operated female and ovariectomized female Wister rats. Estrogen replacement therapy (17-beta -estradiol, 20-100 mug/kg/day, s.c.) has been performed in ovariectomized rats over two weeks. We found that cNOS activity was higher in the aorta and heart of female rats compared to males. Ovariectomy decreased cNOS activity in both tissues to that level what could be observed in males. Estrogen supplementation caused a dose-dependent elevation of cNOS enzyme activity in cardiac and aortic tissues, where the higher dose (100 mug) completely restored cNOS enzyme activity to the levels found in females. We concluded that endogenous estrogens up-regulate the activity of the cNOS isoenzymes in the rat aorta and heart. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：49 / 55

页数：7

共 31 条

[1] INHIBITION OF CORONARY-ARTERY ATHEROSCLEROSIS BY 17-BETA ESTRADIOL IN OVARIECTOMIZED MONKEYS - LACK OF AN EFFECT OF ADDED PROGESTERONE [J].