A novel method to assay herpes simplex virus neutralizing antibodies using BHKICP6LacZ-5 (ELVIS™) cells

被引：8

作者：

Ashley, RL

Dalessio, J

Sekulovich, RE

机构：

[1] Univ Washington, Seattle, WA 98195 USA

[2] Chiron Vaccine Corp, Emeryville, CA USA

来源：

VIRAL IMMUNOLOGY | 1997年 / 10卷 / 04期

关键词：

D O I：

10.1089/vim.1997.10.213

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A novel method for determining neutralizing serum antibody titers to herpes simplex virus type 2 (HSV-2) was developed based on reduction of infectivity in BHKICP6LacZ-5 (ELVIS(TM)) cells; baby hamster kidney (BHK) cells that have been genetically engineered to contain the Escherichia coli LacZ gene under the control of an inducible herpes simplex virus type I (HSV-1) promoter. The test has a semiautomated, colorimetric readout resulting in rapid, objective readings of infectivity reduction. Extent of neutralization is calculated against a calibration curve of virus infectivity generated in each run. HSV-2 neutralizing activity can be detected with serum dilutions in excess of 1:5120.

引用

页码：213 / 220

页数：8

共 30 条

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[2] DETECTION OF TYPE-SPECIFIC ANTIBODY TO HERPES-SIMPLEX VIRUS TYPE-1 BY RADIOIMMUNOASSAY WITH HERPES-SIMPLEX VIRUS TYPE-1 GLYCOPROTEIN-C PURIFIED WITH MONOCLONAL-ANTIBODY [J].