Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples

被引：95

作者：

Leuko, S

Legat, A

Fendrihan, S

Stan-Lotter, H

机构：

[1] Salzburg Univ, Dept Microbiol, Div Mol Biol, A-5020 Salzburg, Austria

[2] Univ New S Wales, Cyanobacteria & Astrobiol Res Lab, Sydney, NSW, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 11期

基金：

奥地利科学基金会;

关键词：

D O I：

10.1128/AEM.70.11.6884-6886.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Extremophilic archaea were stained with the LIVE/DEAD BacLight kit under conditions of high ionic strength and over a pH range of 2.0 to 9.3. The reliability of the kit was tested with haloarchaea following permeabilization of the cells. Microorganisms in hypersaline environmental samples were detectable with the kit, which suggests its potential application to future extraterrestrial halites.

引用

页码：6884 / 6886

页数：3

共 32 条

[1] Fluorescence in situ hybridization analysis of the prokaryotic community inhabiting crystallizer ponds [J].