Trypanocidal activity and acute toxicity assessment of triterpene acids

被引：35

作者：

Ferreira, Daniele da Silva ^{[1
]}

Esperandim, Viviane Rodrigues ^{[1
]}

Alonso Toldo, Miriam Paula ^{[1
]}

Saraiva, Juliana ^{[1
]}

Cunha, Wilson Roberto ^{[2
]}

de Albuquerque, Sergio ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Ciencias Farmaceut, BR-14040903 Ribeirao Preto, SP, Brazil

[2] Univ Franca, Nucleo Pesquisa Ciencias Exatas & Tecnol, BR-14404600 Franca, SP, Brazil

来源：

PARASITOLOGY RESEARCH | 2010年 / 106卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

URSOLIC ACID; OLEANOLIC ACID; RAT HEPATOCYTES; CHAGAS-DISEASE; ORAL TOXICITY; LIVER-DAMAGE; IN-VITRO; MICE; DERIVATIVES; INHIBITION;

D O I：

10.1007/s00436-010-1740-2

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The present study evaluates the in vitro and in vivo trypanocidal activity of ursolic acid and oleanolic acid against the Bolivia strain of Trypanosoma cruzi. Their acute toxicity is also assessed on the basis of median lethal dose (DL50) determination and quantification of biochemical parameters. Ursolic acid is the most active compound in vitro, furnishing IC50 of 25.5 mu M and displaying 77% of trypomastigote lysis at a concentration of 128 A mu M. In agreement with in vitro assays, the results obtained for the in vivo assay reveals that ursolic acid (at a dose of 20 mg/Kg/day) provides the most significant reduction in the number of parasites at the parasitemic peak. Results concerning the LD50 assay and the biochemical parameters evaluated in the present study demonstrate that these substances can be safely used on an experimental basis.

引用

页码：985 / 989

页数：5

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