Cholesterol Sulfate Imaging in Human Prostate Cancer Tissue by Desorption Electrospray Ionization Mass Spectrometry

被引：146

作者：

Eberlin, Livia S. ^{[1
,2
]}

Dill, Allison L. ^{[1
,2
]}

Costa, Anthony B. ^{[1
,2
]}

Ifa, Demian R. ^{[1
,2
]}

Cheng, Liang ^{[4
]}

Masterson, Timothy ^{[5
]}

Koch, Michael ^{[5
]}

Ratliff, Timothy L. ^{[3
]}

Cooks, R. Graham ^{[1
,2
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

[2] Purdue Univ, Ctr Analyt Instrumentat Dev, W Lafayette, IN 47907 USA

[3] Purdue Univ, Ctr Canc Res, W Lafayette, IN 47907 USA

[4] Indiana Univ, Sch Med, Dept Pathol & Lab Med, Indianapolis, IN 46202 USA

[5] Indiana Univ, Sch Med, Dept Urol, Indianapolis, IN 46202 USA

来源：

ANALYTICAL CHEMISTRY | 2010年 / 82卷 / 09期

基金：

美国国家卫生研究院;

关键词：

CHOLINE PHOSPHOLIPID-METABOLISM; CELL CARCINOMA; DIFFERENTIATION; MARKERS;

D O I：

10.1021/ac9029482

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Development of methods for rapid distinction between cancerous and non-neoplastic tissues is an important goal in disease diagnosis. To this end, desorption electrospray ionization mass spectrometry (DESI-MS) imaging was applied to analyze the lipid profiles of thin tissue sections of 68 samples of human prostate cancer and normal tissue. The disease state of the tissue sections was determined by independent histopathological examination. Cholesterol sulfate was identified as a differentiating compound, found almost exclusively in cancerous tissues including tissue containing precancerous lesions. The presence of cholesterol sulfate in prostate tissues might serve as a tool for prostate cancer diagnosis although confirmation through larger and more diverse cohorts and correlations with clinical outcome data is needed.

引用

页码：3430 / 3434

页数：5

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