Glutamine synthetase gene expression at elevated hydrostatic pressure in a deep-sea piezophilic Shewanella violacea

被引：13

作者：

Ikegami, A

Nakasone, K ^{[1
]}

Kato, C

Nakamura, Y

Yoshikawa, I

Usami, R

Horikoshi, K

机构：

[1] Japan Marine Sci & Technol Ctr, DEEPSTAR Grp, Yokosuka, Kanagawa 2370061, Japan

[2] Toyo Univ, Fac Engn, Dept Appl Chem, Kawagoe, Saitama 3500852, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 192卷 / 01期

关键词：

piezophilic bacterium; hydrostatic pressure; glnA operon; sigma(54)-dependent promoter; sigma(70)-dependent promoter; Shewanella violacea;

D O I：

10.1016/S0378-1097(00)00414-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A glutamine synthetase gene (glnA) was isolated from a deep-sea piezophilic bacterium, Shewanella violacea strain DSS12. A 7.5-kb SacI fragment containing the complete glnA gene was cloned and sequenced. The glnA gene was found to encode a protein consisting of 469 amino acid residues, showing 75.0% identity to the glutamine synthetase of Escherichia coli. Primer extension analyses revealed two transcription initiation sites in glnA and expression from each site was positively regulated by pressure. Putative promoters recognized by sigma (70) and sigma (54) were identified in the region upstream of glnA. An electrophoretic mobility shift assay demonstrated that S. violacea sigma (54) specifically binds to the promoter region of glnA, suggesting that sigma (54) may play an important role in pressure-regulated transcription in this piezophilic bacterium. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：91 / 95

页数：5

共 17 条

[1] POSITIVE AND NEGATIVE CONTROL OF THE GLNA-NTRBC REGULON IN KLEBSIELLA-PNEUMONIAE [J].