Depletion of intracellular zinc from neurons by use of an extracellular chelator in vivo and in vitro

被引:63
作者
Frederickson, CJ
Suh, SW
Koh, JY
Cha, YK
Thompson, RB
LaBuda, CJ
Balaji, RV
Cuajungco, MP
机构
[1] NeuroBioTex Inc, Galveston, TX 77550 USA
[2] Univ Ulsan, Coll Med, Natl Creat Res Initiat Ctr Study CNS Zinc, Seoul, South Korea
[3] Univ Maryland, Sch Med, Dept Biochem, Baltimore, MD 21201 USA
[4] Harvard Univ, Sch Med, Inst Human Genet, Boston, MA 02115 USA
[5] Massachusetts Gen Hosp, Mol Neurogenet Unit, Charlestown, MA USA
关键词
zinc; hippocampus; nitric oxide; TSQ; (N-(6-methoxy-8-quinolyl)-para-toluenesulfonamide); neurotoxicity; CaEDTA;
D O I
10.1177/002215540205001210
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The membrane-impermeable chelator CaEDTA was introduced extracellularly among neurons in vivo and in vitro for the purpose of chelating extracellular Zn2+. Unexpectedly, this treatment caused histochemically reactive Zn2+ in intracellular compartments to drop rapidly. The same general result was seen with intravesicular Zn2+, which fell after CaEDTA infusion into the lateral ventricle of the brain, with perikaryal Zn2+ in Purkinje neurons (in vivo) and with cortical neurons (in vitro). These findings suggest either that the volume of zinc ion efflux and reuptake is higher than previously suspected or that EDTA can enter cells and vesicles. Caution is therefore warranted in attempting to manipulate extracellular or intracellular Zn2+ selectively.
引用
收藏
页码:1659 / 1662
页数:4
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