Cytoskeletal requirements for hepatitis C virus (HCV) RNA synthesis in the HCV replicon cell culture system

被引：66

作者：

Bost, AG ^{[1
]}

Venable, D ^{[1
]}

Liu, L ^{[1
]}

Heinz, BA ^{[1
]}

机构：

[1] Lilly Corp Ctr, Eli Lilly & Co, Lilly Res Labs, Infect Dis Res, Indianapolis, IN 46285 USA

来源：

JOURNAL OF VIROLOGY | 2003年 / 77卷 / 07期

关键词：

D O I：

10.1128/JVI.77.7.4401-4408.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis C virus (HCV) induces microtubule aggregates in infected hepatocytes. To determine if cytoskelletall elements are important for HCV RNA synthesis, we examined the effect of cytoskeleton inhibitors on HCV replicon transcription in Huh7 cells. The data demonstrate that HCV replication complex-mediated RNA synthesis requires microtubule and actin polymerization.

引用

页码：4401 / 4408

页数：8

共 37 条

[1] Efficient initiation of HCV RNA replication in cell culture
Blight, KJ
Kolykhalov, AA
Rice, CM
[J]. SCIENCE, 2000, 290 (5498) : 1972 - 1974
[2] POSTTRANSFUSION NON-A, NON-B HEPATITIS IN CHIMPANZEES - PHYSICOCHEMICAL EVIDENCE THAT THE TUBULE-FORMING AGENT IS A SMALL, ENVELOPED VIRUS
BRADLEY, DW
MCCAUSTLAND, KA
COOK, EH
SCHABLE, CA
EBERT, JW
MAYNARD, JE
[J]. GASTROENTEROLOGY, 1985, 88 (03) : 773 - 779
[3] An amino-terminal amphipathic α-helix mediates membrane association of the hepatitis C virus nonstructural protein 5A
Brass, V
Bieck, E
Montserret, R
Wölk, B
Hellings, JA
Blum, HE
Penin, F
Moradpour, D
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (10) : 8130 - 8139
[4] ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME
CHOO, QL
KUO, G
WEINER, AJ
OVERBY, LR
BRADLEY, DW
HOUGHTON, M
[J]. SCIENCE, 1989, 244 (4902) : 359 - 362
[5] Golgi dispersal during microtubule disruption: Regeneration of Golgi stacks at peripheral endoplasmic reticulum exit sites
Cole, NB
Sciaky, N
Marotta, A
Song, J
LippincottSchwartz, J
[J]. MOLECULAR BIOLOGY OF THE CELL, 1996, 7 (04) : 631 - 650
[6] DePina AS, 1999, MICROSC RES TECHNIQ, V47, P93, DOI 10.1002/(SICI)1097-0029(19991015)47:2<93::AID-JEMT2>3.0.CO
[7] 2-P
[8] Hepatitis C virus glycoprotein complex localization in the endoplasmic reticulum involves a determinant for retention and not retrieval
Duvet, S
Cocquerel, L
Pillez, A
Cacan, R
Verbert, A
Moradpour, D
Wychowski, C
Dubuisson, J
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (48) : 32088 - 32095
[9] Expression of hepatitis C virus proteins induces distinct membrane alterations including a candidate viral replication complex
Egger, D
Wölk, B
Gosert, R
Bianchi, L
Blum, HE
Moradpour, D
Bienz, K
[J]. JOURNAL OF VIROLOGY, 2002, 76 (12) : 5974 - 5984
[10] Expression of hepatitis C virus cDNA in human hepatoma cell line mediated by a hybrid baculovirus-HCV vector
Fipaldini, C
Bellei, B
La Monica, N
[J]. VIROLOGY, 1999, 255 (02) : 302 - 311

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