Cloning and functional expression of human retinal Kir2.4, a pH-sensitive inwardly rectifying K+ channel

被引：42

作者：

Hughes, BA

Kumar, G

Yuan, Y

Swaminathan, A

Yan, D

Sharma, A

Plumley, L

Yang-Feng, TL

Swaroop, A

机构：

[1] Univ Michigan, Kellogg Eye Ctr, Dept Ophthalmol, Ann Arbor, MI 48105 USA

[2] Univ Michigan, Dept Physiol, Ann Arbor, MI 48105 USA

[3] Univ Michigan, Dept Human Genet, Ann Arbor, MI 48105 USA

[4] Yale Univ, Dept Genet, New Haven, CT 06520 USA

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2000年 / 279卷 / 03期

关键词：

potassium channels; nucleotide sequence; chromosomal localization;

D O I：

10.1152/ajpcell.2000.279.3.C771

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To identify novel potassium channel genes expressed in the retina, we screened a human retina cDNA library with an EST sequence showing partial homology to inwardly rectifying potassium (Kir) channel genes. The isolated cDNA yielded a 2,961-base pair sequence with the predicted open reading frame showing strong homology to the rat Kir2.4 (rKir2.4). Northern analysis of mRNA from human and bovine tissues showed preferential expression of Kir2.4 in the neural retina. In situ hybridization to sections of monkey retina detected Kir2.4 transcript in most retinal neurons. Somatic hybridization analysis and dual-color in situ hybridization to metaphase chromosomes mapped Kir2.4 to human chromosome 19 q13.1-q13.3. Expression of human Kir2.4 cRNA in Xenopus oocytes generated strong, inwardly rectifying K+ currents that were enhanced by extracellular alkalinization. We conclude that human Kir2.4 encodes an inwardly rectifying K+ channel that is preferentially expressed in the neural retina and that is sensitive to physiological changes in extracellular pH.

引用

页码：C771 / C784

页数：14

共 56 条

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