Suppression of the inflammatory response from adherent cells on phospholipid polymers

被引:89
作者
Sawada, S
Sakaki, S
Iwasaki, Y
Nakabayashi, N
Ishihara, K
机构
[1] Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Div Organ Mat, Chiyoda Ku, Tokyo 1010062, Japan
[2] Univ Tokyo, Sch Engn, Dept Mat Engn, Bunkyo Ku, Tokyo 1138656, Japan
来源
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2003年 / 64A卷 / 03期
关键词
messenger RNA (mRNA) expression; reverse transcription polymerase chain reaction (RT-PCR); interleukin-1 beta (IL-1 beta); phospholipid polymer; biocompatibility;
D O I
10.1002/jbm.a.10433
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The expression of interleukin-1beta (IL-1beta) messenger RNA (mRNA) in macrophage-like cells cultured on phospholipid polymers was evaluated to determine the extent of the inflammatory response. As phospholipid polymers, poly(2-methacryloyloxyethyl phosphorylcholine(MPC)-co-n-butyl methacrylate(BMA)s (PMBs) were synthesized. Poly(ethylene terephthalate) (PET), poly(2hydroxyethyl methacrylate) (PHEMA), and segmented poly(ether urethane) (Tecoflex(R) 60) were used as reference biomedical polymers. The protein adsorption onto the polymer surfaces from a cell culture medium was determined. The amount of the total protein adsorbed onto the PMBs was lower than that adsorbed onto the reference polymers, and the amount of adsorbed protein decreased with an increase in the MPC units in the PMBs. Human premyelocytic leukemia cell line (HL-60) was used, and the expression of IL-1beta mRNA was investigated with the reverse transcription polymerase chain reaction (RT-PCR) method. When HL-60 cells were cultured on PMBs, the expression of IL-1beta mRNA in the cells was much less than that on the reference polymers. In particular, the expression of IL-1beta mRNA in HL-60 cells cultured on the PMBs containing more than 10 mol % MPC units was not detected. This corresponded to the reduced amount of adsorbed proteins on the PMB surfaces. These results suggest that the PMBs effectively suppressed the activation and inflammatory response of adherent macrophagelike cells. (C) 2003 Wiley Periodicals, Inc.
引用
收藏
页码:411 / 416
页数:6
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