Prospective value of PCR amplification and sequencing for diagnosis and typing of Old World Leishmania infections in an area of nonendemicity

被引:36
作者
Gangneux, JP
Menotti, J
Lorenzo, F
Sarfati, C
Blanche, H
Bui, H
Pratlong, F
Garin, YJF
Derouin, F
机构
[1] Ctr Hosp Univ Rennes, Lab Parasitol Mycol, F-35000 Rennes, France
[2] Ctr Hosp Univ Lariboisiere St Louis, Lab Parasitol Mycol, F-75010 Paris, France
[3] Hop St Louis, Ctr Etud Polymorphisme Humain, F-75475 Paris, France
[4] Lab Ecol Med & Pathol Parasitaire, F-34090 Montpellier, France
关键词
D O I
10.1128/JCM.41.4.1419-1422.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We assessed the prospective value of PCR amplification of a repetitive sequence from Leishmania nuclear DNA and sequencing for the diagnosis and typing of Old World Leishmania infection in an area of nonendemicity. During this 42-month study, 29 of 168 consecutive samples were examined and classified as positive for Leishmania by direct examination and/or in vitro culture. This molecular approach showed excellent sensitivity (97%) and specificity (100%) compared to direct examination (86 and 100%, respectively) and in vitro culture (72 and 100%, respectively). Isoenzymatic and molecular typing allowed similar identification for 12 samples. Besides, PCR and subsequent sequencing of DNA products permitted the species identification of 14 samples for which parasite culture remained negative or did not allow isoenzymatic characterization, indicating the complementarity of parasitological and molecular tools.
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页码:1419 / 1422
页数:4
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