Preparation protocols for high-activity Photosystem II membrane particles of green algae and higher plants, pH dependence of oxygen evolution and comparison of the S2-state multiline signal by X-band EPR spectroscopy

被引：87

作者：

Schiller, H

Dau, H

机构：

[1] Univ Marburg, FB Biol, D-35032 Marburg, Germany

[2] Free Univ Berlin, FB Phys, D-14195 Berlin, Germany

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2000年 / 55卷 / 2-3期

关键词：

manganese; oxygen-evolving complex; Chlamydomonas reinhardtii; Scenedesmus obliquus; photosynthesis;

D O I：

10.1016/S1011-1344(00)00036-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Photosystem II (PS II) membrana particles are particularly well suited for various types of spectroscopic investigations on the PS II manganese complex. Here we present: ( I) a preparation protocol for PS II membrane particles of higher plants, which yields exceptionally high oxygen-evolution activity due to the use of glycinebetaine as a PS II-stabilizing agent; (2) preparation protocols for highly active PS II membrane particles for the green algae Scenedesmus obliquus and Chlamydomonas reinhardtii; (3) a determination of pH dependence of oxygen evolution for spinach and Scenedesmus; (4) a comparison of the EPR multiline signal observed in the S-2-state of green algae and higher plants of PS II membrane particles. A clearly broader type of multiline EPR signal is observed in green algae. (C)2000 Elsevier Science S.A. All rights reserved.

引用

页码：138 / 144

页数：7

共 39 条

[1] ISOLATION AND CHARACTERIZATION OF THE PHOTOSYSTEM 2 REACTION-CENTER COMPLEX FROM A DOUBLE MUTANT OF CHLAMYDOMONAS-REINHARDTII [J].