Demonstration of In vivo expression of a hypothetical open reading frame (ORF-14) encoded by the RD1 region of Mycobacterium tuberculosis

被引：14

作者：

Amoudy, H. A.

Ahmad, S.

Thole, J. E.

Mustafa, A. S.

机构：

[1] Kuwait Univ, Fac Med, Dept Microbiol, Safat 13110, Kuwait

[2] Univ London Imperial Coll Sci Technol & Med, Dept Microbiol & Immunol, London, England

[3] Anim Sci Grp, Div Infect Dis, Lelystad, Netherlands

来源：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 2007年 / 66卷 / 04期

关键词：

D O I：

10.1111/j.1365-3083.2007.01961.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Previously we identified a novel antigenic open reading frame (ORF), designated as ORF-14, on the RD1 region of Mycobacterium tuberculosis that was not originally predicted by Mahairas or by annotation of the M. tuberculosis H37 Rv genome. Here we show that anti-ORF-14 antibodies either from mice immunized with recombinant ORF-14 protein or isolated from serum samples from tuberculosis patients, react with a protein in culture filtrate but not in cytoplasmic or cell wall fractions from M. tuberculosis. Our data indicate that the ORF-14 protein is expressed as a secreted protein, representing one more secreted protein antigen not previously identified by genomics.

引用

页码：422 / 425

页数：4

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