Molecular cloning and analysis of expression of the Leishmania infantum histone H4 genes

被引：29

作者：

Soto, M ^{[1
]}

Quijada, L ^{[1
]}

Alonso, C ^{[1
]}

Requena, JM ^{[1
]}

机构：

[1] Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1997年 / 90卷 / 02期

关键词：

Leishmania infantum; histone H4; expression; aphidicolin; hydroxyurea; DNA synthesis;

D O I：

10.1016/S0166-6851(97)00178-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present work, we describe the sequence, organization and expression of histone H4 genes in the protozoan parasite Leishmania infantum. The predicted L. infantum histone H4 is a polypeptide of 100 amino acids with a molecular mass of 11.5 kDa. Comparison of the amino acid sequence of Leishmania histone H4 with the rest of histone H4 sequences indicates that this is the most divergent sequence reported to date. The genomic distribution analysis of histone H4 genes indicates that there must be up to seven gene copies. A single size-class histone H4 mRNA of 0.6 kb was detected, whose level dramatically decreases from logarithmic to stationary phase. However, the Leishmania histone H4 mRNAs do not decrease in abundance following treatment with inhibitors of DNA synthesis,suggesting a regulation by a replication-independent mechanism. (C) 1997 Elsevier Science B.V.

引用

页码：439 / 447

页数：9

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