Rabbit extracellular superoxide dismutase:: expression and effect on LDL oxidation

被引：40

作者：

Laukkanen, MO

Lehtolainen, P

Turunen, P

Aittomäki, S

Oikari, P

Marklund, SL

Ylä-Herttuala, S

机构：

[1] Univ Kuopio, AI Virtanen Inst Mol Sci, FIN-70211 Kuopio, Finland

[2] Univ Kuopio, Dept Med, FIN-70211 Kuopio, Finland

[3] Univ Tampere, Inst Med Technol, FIN-33101 Tampere, Finland

[4] Univ Umea Hosp, Dept Med Biosci, S-90185 Umea, Sweden

来源：

GENE | 2000年 / 254卷 / 1-2期

基金：

芬兰科学院;

关键词：

adenovirus; antioxidant; EC-SOD; post-transcriptional regulation;

D O I：

10.1016/S0378-1119(00)00272-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Extracellular superoxide dismutase (EC-SOD) is a secreted antioxidative enzyme with an abundant mRNA expression in kidney and arterial wail. In order to study expression and antioxidative function of EC-SOD, we cloned the rabbit ec-sod cDNA and produced the recombinant protein in cell culture. In vitro studies did not show a direct relationship between the amounts of synthesized mRNA and secreted protein activity, suggesting post-transcriptional regulation. The antiatherogenic role of EC-SOD was studied by determining the effect of EC-SOD on the oxidation (ox) of low density lipoprotein (LDL), and subsequent degradation of oxLDL in RAW 264 macrophages in vitro. It was found that resombinant EC-SOD reduced both the degradation of LDL in RAW 264 macrophages by 28-36% and its electrophoretic mobility caused by endothelial cell-mediated oxidation. It is therefore suggested that EC-SOD can act as a protective enzyme against the development of atherosclerosis. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：173 / 179

页数：7

共 40 条

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