Cytotoxicity of xenobiotics and expression of glutathione-S-transferases in immortalised rat hepatocyte cell lines

被引：9

作者：

Anderson, K

Andrews, R

Yin, L

McLeod, R

MacDonald, C

Hayes, JD

Grant, MH ^{[1
]}

机构：

[1] Univ Strathclyde, Wolfson Ctr, Bioengn Unit, Glasgow G4 0NW, Lanark, Scotland

[2] Univ Dundee, Ninewells Hosp & Med Sch, Biomed Res Ctr, Dundee DD1 9SY, Scotland

[3] Univ Paisley, Paisley PA1 2BE, Renfrew, Scotland

来源：

HUMAN & EXPERIMENTAL TOXICOLOGY | 1998年 / 17卷 / 03期

关键词：

immortalised hepatocytes; glutathione S-transferases; cytotoxicity;

D O I：

10.1191/096032798678908404

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

1 Immortalised rat hepatocyte cell lines are more sensitive to the cytotoxicity of 1-chloro-2,4-dinitrobenzene and ethacrynic acid than primary cultures of hepatocytes. 2 Class alpha glutathione S-transferases are not expressed in immortalised hepatocyte cell lines. Class pi glutathione S-transferase expression is elevated in the immortalised cell lines compared with freshly isolated hepatocytes, but it is not as high as in the HTC rat hepatoma cell line. 3 Immortalised hepatocyte cell lines may provide a sensitive model system for detecting cytotoxicity associated with xenobiotics which are detoxified by glutathione S-transferases.

引用

页码：131 / 137

页数：7

共 35 条

[1] Immortalized hepatocytes as in vitro model systems for toxicity testing: The comparative toxicity of menadione in immortalized cells, primary cultures of hepatocytes and HTC hepatoma cells [J].