Antioxidant actions of ovothiol-derived 4-mercaptoimidazoles: glutathione peroxidase activity and protection against peroxynitrite-induced damage

被引:47
作者
Bailly, F
Zoete, V
Vamecq, J
Catteau, JP
Bernier, JL
机构
[1] Univ Lille 1, Chim Organ Phys Lab, CNRS, ESA 8009, F-59655 Villeneuve Dascq, France
[2] Hop Robert Debre, INSERM, E9935, F-75019 Paris, France
关键词
4-mercaptoimidazole; ovothiol; glutathione peroxidase; peroxynitrite; antioxidant;
D O I
10.1016/S0014-5793(00)02234-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
4-Mercaptoimidazoles derived from the naturally occurring antioxidants, ovothiols, were tested for their glutathione peroxidase-like (GSH Px-like) activity and protection against peroxynitrite-induced damage. All the thiol compounds displayed similar significant GSH Px-like activities, which are however weaker than that of the reference compound, ebselen, The inhibitions of the peroxynitrite-dependent oxidation of Evans blue dye and dihydrorhodamine 123 showed that the thiol compounds substituted on position 5 of the imidazole ring were nearly as effective as ebselen while the C-2 substituted ones were less effective, Both assays corroborate the large superiority of mercaptoimidazoles over glutathione as inhibitors of peroxynitrite-dependent oxidation, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V, All rights reserved.
引用
收藏
页码:19 / 22
页数:4
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