From observed to corrected luminescence intensity of solution systems: an easy-to-apply correction method for standard spectrofluorimeters

Although spectrofluorimetry is a very sensitive analytical technique, its use is limited by the non linear relationship between the concentration of the analyte of interest and the generated electric signal. Here we discuss a very easy correction method which takes into account all the instrumental factors and transforms the observed intensity value in a quantity, the corrected luminescence intensity, that is directly proportional to the concentration of the observed luminophore. At the end of the discussion, a general formula is presented together with two examples illustrating the method and its application. (C) 1998 Elsevier Science B.V.