Dentin-derived BMP-2 and Odontoblast Differentiation

被引：228

作者：

Casagrande, L. ^{[1
,2
]}

Demarco, F. F. ^{[1
,3
]}

Zhang, Z. ^{[1
]}

Araujo, F. B. ^{[2
]}

Shi, S. ^{[4
]}

Nor, J. E. ^{[1
,5
,6
]}

机构：

[1] Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Angiogenesis Res Lab, Ann Arbor, MI 48109 USA

[2] Univ Fed Rio Grande do Sul, Sch Dent, Dept Pediat Dent, Porto Alegre, RS, Brazil

[3] Univ Fed Pelotas, Sch Dent, Dept Operat Dent, Pelotas, Brazil

[4] Univ So Calif, Sch Dent, Ctr Craniofacial Mol Biol, Los Angeles, CA 90089 USA

[5] Univ Michigan, Coll Engn, Dept Biomed Engn, Ann Arbor, MI 48109 USA

[6] Univ Michigan, Sch Med, Dept Otolaryngol, Ann Arbor, MI 48109 USA

来源：

JOURNAL OF DENTAL RESEARCH | 2010年 / 89卷 / 06期

关键词：

endodontics; tissue engineering; stem cells; dental pulp; trauma; BONE MORPHOGENETIC PROTEIN-2; EXFOLIATED DECIDUOUS TEETH; STEM-CELLS; PULP CELLS; IN-VITRO; OSTEOGENIC PROTEIN-1; EXPRESSION PATTERNS; GENE-EXPRESSION; MATRIX; SIALOPROTEIN;

D O I：

10.1177/0022034510364487

中图分类号：

R78 [口腔科学];

学科分类号：

100302 [口腔临床医学];

摘要：

It is known that stem cells from exfoliated deciduous teeth (SHED) can be induced to differentiate into odontoblasts. However, the nature of dentin-derived morphogenic signals required for dental pulp stem cell differentiation remains unclear. The hypothesis underlying this work is that dentin-derived Bone Morphogenetic Proteins (BMP) are necessary for the differentiation of SHED into odontoblasts. We observed that SHED express markers of odontoblastic differentiation (DSPP, DMP-1, MEPE) when seeded in human tooth slice/scaffolds and cultured in vitro, or implanted subcutaneously into immunodeficient mice. In contrast, SHED cultured in deproteinized tooth slice/scaffolds, or scaffolds without a tooth slice, do not express these markers. SHED express the BMP receptors BMPR-IA, BMPR-IB, and BMPR-II. Notably, blockade of BMP-2 signaling inhibited the expression of markers of odontoblastic differentiation by SHED cultured in tooth slice/scaffolds. Collectively, this work demonstrates that dentin-derived BMP-2 is required to induce the differentiation of SHED into odontoblasts.

引用

页码：603 / 608

页数：6

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