Evaluation of a direct immunofluorescence cytospin assay for the detection of herpes simplex virus in clinical samples

被引：13

作者：

Reina, J ^{[1
]}

Saurina, J ^{[1
]}

Fernandez-Baca, V ^{[1
]}

Munar, M ^{[1
]}

Blanco, I ^{[1
]}

机构：

[1] Univ Hosp Son Dureta UIB, Clin Microbiol Serv, Virol Unit, E-07014 Palma de Mallorca, Spain

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 1997年 / 16卷 / 11期

关键词：

D O I：

10.1007/BF01700419

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A comparison between a direct immunofluorescence assay (DFA) and the shell-vial culture (SVC) was conducted to evaluate their efficacies according to the quality and origin of the sample and the type of herpes simplex (HSV) responsible for the infection, The SVC detected all 58 HSV-infected samples, while the DFA detected only 49 (84.5%) positive samples, The DFA detected HSV type 1 in 22 of 89 samples (24.7%) and HSV type 2 in 27 of 96 samples (28.1%), Compared with the SVC, the DFA had a sensitivity of 75.8% for HSV type 1 and 93.1% for HSV type 2, The sensitivity of the DFA depends on the quality of the sample, Thus, while DFA is recommendable as a screening method, the SVC remains the method of choice for obtaining the maximum diagnostic yield from the sample.

引用

页码：851 / 854

页数：4

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