Novel and variant ribozymes obtained through in vitro selection

Ribozymes are RNA molecules capable of catalyzing chemical reactions. Natural ribozymes generally accelerate the rate of cleavage and ligation of specific phosphodiester bonds. In vitro selection of RNA is now being used as a powerful technique to isolate novel and variant ribozymes that carry out catalysis at phosphodiester and carbon bonds. The range of reactions catalyzed by in vitro selected ribozymes is now well beyond the scope of known natural ribozymes.