A time-lapse capillary assay to study aerotaxis in the archaeon Halobacterium salinarum

被引：5

作者：

Boudko, D

Yu, HS

Ruiz, M

Hou, S

Alam, M

机构：

[1] Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA

[2] Univ Florida, Whitney Lab, St Augustine, FL 32080 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2003年 / 53卷 / 01期

基金：

美国国家科学基金会;

关键词：

optical density; Halobacterium salinarum; cells;

D O I：

10.1016/S0167-7012(02)00227-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a method for time-lapse video photography and line scanning of optical densities for analysis of aerotactic responses of Halobacterium salinarum. This automated digital technique, along with line scan analysis of selected frames, gives a unique profile of the aerotactic migration of halobacterial cells. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：123 / 126

页数：4

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