Enantiomer separations by capillary electrochromatography using chiral stationary phases

被引：63

作者：

Otsuka, K ^{[1
]}

Mikami, C ^{[1
]}

Terabe, S ^{[1
]}

机构：

[1] Himeji Inst Technol, Fac Sci, Dept Mat Sci, Kamigori, Hyogo 6781297, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 887卷 / 1-2期

关键词：

chiral stationary phases; CEC; enantiomer separation;

D O I：

10.1016/S0021-9673(99)01205-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The applicability of capillary electrochromatography (CEC) using packed capillary column to enantiomer separations was investigated. As chiral stationary phases, OD type packing materials of 5 and 3 mu m particle diameters, originally designed for conventional high-performance liquid chromatography (HPLC) were employed. The chiral packing materials were packed by a pressurized method into a 100 mu m I.D. fused-silica capillary. Several racemic enantiomers, such as acidic, neutral and basic drug components, were successfully resolved, typically by using acidic or basic solutions containing acetonitrile as mobile phases. The separation efficiencies for some enantiomers in the chiral CEC system using the 5 mu m OD type packing were superior to those obtained in HPLC using chiral packings. The plate heights obtained for several enantiomers were 8-13 mu m or the reduced plate height of 1.6-2.6, which indicates the high efficiency of this chiral CEC system. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：457 / 463

页数：7

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