Mammalian thioredoxin reductase alters cytolytic activity of an antibacterial peptide

被引：14

作者：

Björkhem-Bergman, L

Jönsson-Videsäter, K

Paul, C

Björnstedt, M

Andersson, M ^{[1
]}

机构：

[1] Karolinska Inst, Ctr Microbiol & Tumor Biol, S-17177 Stockholm, Sweden

[2] Huddinge Univ Hosp, Karolinska Inst, Dept Lab Med, Div Pathol, S-14186 Stockholm, Sweden

[3] Huddinge Univ Hosp, Karolinska Inst, Dept Med, Div Hematol & Oncol, S-14186 Stockholm, Sweden

来源：

PEPTIDES | 2004年 / 25卷 / 11期

关键词：

granulysin; protegrin; defensin; thioredoxin reductase; thioredoxin; glutaredoxin;

D O I：

10.1016/j.peptides.2004.06.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Granulysin is a disulfide rich 9 kDa human tumoricidal protein produced by cytolytic cells. Here we show that thioredoxin reductase (TrxR) reduced a 23-residue peptide from granulysin (GranF2), and this markedly enhanced the killing of small cell lung cancer cells (SCLC) by GranF2. Cells treated with reduced GranF2 showed rapid ATP deletion within 90 min and strong annexin V staining after 4 h incubation. SCLC with elevated TrxR levels was more sensitive to oxidized GranF2 than normal cells. The levels of TrxR are enhanced in many cancer cells, including SCLC, and it is possible that cytolytic activity of cytolytic cells on SCLC may in part be mediated by granulysin and modulated by TrxR. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：1849 / 1855

页数：7

共 40 条

[1] Granulysin crystal structure and a structure-derived lytic mechanism [J].