Evaluation of major active components in St. John's Wort dietary supplements by high-performance liquid chromatography with photodiode array detection and electrospray mass spectrometric confirmation

被引：70

作者：

Liu, FF ^{[1
]}

Ang, CYW ^{[1
]}

Heinze, TM ^{[1
]}

Rankin, JD ^{[1
]}

Beger, RD ^{[1
]}

Freeman, JP ^{[1
]}

Lay, JO ^{[1
]}

机构：

[1] US FDA, Natl Ctr Toxicol Res, Div Chem, Jefferson, AR 72079 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 888卷 / 1-2期

关键词：

food analysis; St. John's Wort; Hypericum peforatum; naphthodianthrones; flavonoids; phloroglucinols;

D O I：

10.1016/S0021-9673(00)00555-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A RP-HPLC method with photodiode array detection and LC-electrospray ionization (ESI) MS confirmation was established for the determination of major active components in St. John's Wort dietary supplement capsules. The samples alternatively were extracted with ethanol-acetone (2:3) using a 55 degrees C water-bath shaker or an ambient temperature ultrasonic bath. Extracts were separated by RP-C-18 chromatography using a 95-min water-methanol-acetonitrile-trifluoroacetic acid gradient. The major components were identified by photodiode array detection and-then confirmed by LC-ESI-MS. The quantification of components was performed using an internal standard (luteolin). This method may serve as a valuable tool for the quality evaluation of St, John's Wort dietary supplement products. (C) 2000 Published by Elsevier Science B.V.

引用

页码：85 / 92

页数：8

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