Postmortem diagnosis of Fusarium panophthalmitis by the polymerase chain reaction

被引:10
作者
Alexandrakis, G [1 ]
Sears, M [1 ]
Gloor, P [1 ]
机构
[1] YALE UNIV,SCH MED,DEPT OPHTHALMOL & VISUAL SCI,NEW HAVEN,CT 06520
关键词
D O I
10.1016/S0002-9394(14)70594-X
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
PURPOSE: We undertook this study to determine if a polymerase chain reaction-based test that we developed for the filamentary fungus, Fusarium, could be used to detect the organism in postmortem ocular tissues. METHODS: We applied the polymerase chain reaction to amplify a target fragment of Fusarium DNA from formalin-fixed ocular tissues from a patient with endogenous Fusarium panophthalmitis. RESULTS: By using the polymerase chain reaction-based test, we were able to amplify the target fragment of DNA from the infected eyes, but not from uninfected control eyes. CONCLUSIONS: The technique appears to hold promise to be a sensitive, specific, and rapid method of diagnosing Fusarium infections.
引用
收藏
页码:221 / 223
页数:3
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