Effect of TGF-β1 on the Migration and Recruitment of Mesenchymal Stem Cells after Vascular Balloon Injury: Involvement of Matrix Metalloproteinase-14

被引：36

作者：

Zhao, Wei ^{[1
,2
]}

Wang, Chengyan ^{[1
,2
]}

Liu, Ruixue ^{[1
,2
]}

Wei, Cuilei ^{[1
,2
]}

Duan, Juncang ^{[3
]}

Liu, Kejian ^{[3
]}

Li, Shugang ^{[4
]}

Zou, Hong ^{[1
,2
]}

Zhao, Jin ^{[1
,2
]}

Wang, Lianghai ^{[1
,2
]}

Qi, Yan ^{[1
,2
]}

Liang, Weihua ^{[1
,2
]}

Jiang, Jinfang ^{[1
,2
]}

Zhang, Wenjie ^{[1
,2
]}

Pang, Lijuan ^{[1
,2
]}

Li, Feng ^{[1
,2
]}

机构：

[1] Shihezi Univ, Sch Med, Dept Pathol, 59 North 2nd Rd, Shihezi 832002, Xinjiang, Peoples R China

[2] Shihezi Univ, Sch Med, Key Lab Xinjiang Endem & Ethn Dis, Minist Educ, 59 North 2nd Rd, Shihezi 832002, Xinjiang, Peoples R China

[3] Shihezi Univ, Sch Med, Dept Cardiol, Affiliated Hosp 1, 45 North 3rd Rd, Shihezi 832008, Xinjiang, Peoples R China

[4] Shihezi Univ, Sch Med, Dept Publ Hlth, 59 North 2nd Rd, Shihezi 832002, Xinjiang, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

中国国家自然科学基金;

关键词：

GROWTH-FACTOR-BETA; SMOOTH-MUSCLE-CELLS; BONE-MARROW; TRANSFORMING GROWTH-FACTOR-BETA-1; STROMAL CELLS; ARTERIAL-WALL; RESTENOSIS; EXPRESSION; MT1-MMP; ATHEROSCLEROSIS;

D O I：

10.1038/srep21176

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Restenosis or occlusion after vascular procedures is ascribed to intimal hyperplasia. Transforming growth factor (TGF)-beta 1 is involved in recruitment of mesenchymal stem cells (MSCs) following arterial injury, and its release from latent TGF-binding protein by matrix metalloproteinase (MMP)-14-induced proteolysis contributes to neointima formation. However, the relationship between MMP-14 and TGF-beta 1 activation in restenosis is unknown. This study investigated the relationship using a rat model of balloon-induced injury. Rats were assigned to vehicle-, SB431542 (SB)-, or recombinant human (rh) TGF-beta 1-treated groups and examined at various time points after balloon-induced injury for expression of TGF-beta 1/Smad signalling pathway components, MMP-14 and MSCs markers including Nestin, CD29, and Sca1(+)CD29(+)CD11b/c(-)CD45(-). Intimal hyperplasia was reduced in SB- and rhTGF-beta 1-treated rats. The expression of TGF-beta 1, TGF-beta 1RI, and Smad2/3 was decreased, but the levels of phosphorylated Smad2/3 were higher in SB-treated rats than vehicle-treated after 7 days to 14 days. rhTGF-beta 1 administration decreased the expression of TGF-beta 1/Smad pathway proteins, except for TGF-beta 1RI. Nestin and CD29 expression and the number of Sca1(+)CD29(+)CD11b(-)CD45(-)cells were reduced, whereas MMP-14 expression was increased after SB431542 and rhTGF-beta 1 administration. These results suggest that TGF-beta 1/Smad signalling and MMP-14 act to recruit MSCs which differentiate to vascular smooth muscle cells and mesenchymal-like cells that participate in arterial repair/remodelling.

引用

页数：11

共 63 条

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