Development of microsatellite markers from an ectomycorrhizal fungus, Tricholoma matsutake, by an ISSR-suppression-PCR method

被引:41
作者
Lian, CL
Hogetsu, T
Matsushita, N
Guerin-Laguette, A
Suzuki, K
Yamada, A
机构
[1] Univ Tokyo, Asian Nat Environm Sci Ctr, Tokyo 1880002, Japan
[2] Univ Tokyo, Grad Sch Agr & Life Sci, Lab Forest Bot, Bunkyo Ku, Tokyo 1138657, Japan
[3] Shinshu Univ, Fac Agr, Dept Biosci & Biotechnol, Nagano 3994598, Japan
关键词
mycorrhizal fungus; population genetics; SSR polymorphism; Tricholoma matsutake;
D O I
10.1007/s00572-002-0193-6
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
An inter-simple sequence repeat (ISSR)-suppression-PCR technique established to develop microsatellite markers of plant species was applied to an ectomycorrhizal fungus, Tricholoma matsutake. Six polymorphic SSR markers were developed. All six polymorphic SSR markers were single-locused and co-dominant. Alleles produced by these six single-locused markers ranged from two to nine per locus and the expected heterozygosities were calculated as values from 0.098 to 0.803. The results indicated that the ISSR-suppression-PCR technique was effective and applicable to the development of microsatellite markers from ectomycorrhizal fungi. Furthermore, the six microsatellite loci did not amplify DNA from any other ectomycorrhizal species investigated, except for Tricholoma nauseosum (Swedish matsutake) and Tricholoma fulvocastaneum, suggesting that population genetics and reproduction of T matsutake could be investigated by the SSR markers developed in the present study.
引用
收藏
页码:27 / 31
页数:5
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