Cloning, sequencing, and expression of the Bombyx mori receptor for Bacillus thuringiensis insecticidal CryIA(a) toxin

被引：92

作者：

Nagamatsu, Y ^{[1
]}

Toda, S ^{[1
]}

Koike, T ^{[1
]}

Miyoshi, Y ^{[1
]}

Shigematsu, S ^{[1
]}

Kogure, M ^{[1
]}

机构：

[1] Hiroshima Univ, Fac Appl Biol Sci, Dept Appl Biochem, Hiroshima 739, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 04期

关键词：

Bacillus thuringiensis; biopesticide; toxin; receptor; cadherin repeat;

D O I：

10.1271/bbb.62.727

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacillus thuringiensis strains produce insect-specific Bt toxins. Bt CryIA(a) toxin binds to a 175-kDa glycoprotein (BtR175) on the microvillus membranes of columnar cells in the Bombyx mori midgut and causes lysis of the cells. BtR175 was purified, and its cDNA was cloned. The cDNA encodes a newly identified 193.3-kDa preproprotein form of BtR175 that includes nine extracellular cadherin repeats, a 23.5-kDa membrane-proximal domain, a membrane-spanning region, and a 13.6-kDa cytoplasmic domain. Spodoptera frugiperda cells transfected with a recombinant baculovirus DNA carrying the cDNA produced a 175-kDa protein that reacted with anti-BtR antibodies and the Bt CryIA(a) toxin.

引用

页码：727 / 734

页数：8

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