Detection of p53 dysfunction by flow cytometry in chronic lymphocytic leukaemia

被引：33

作者：

Carter, A ^{[1
]}

Lin, K ^{[1
]}

Sherrington, PD ^{[1
]}

Pettitt, AR ^{[1
]}

机构：

[1] Royal Liverpool Univ Hosp, Dept Haematol, Liverpool L7 8XP, Merseyside, England

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 2004年 / 127卷 / 04期

关键词：

chronic lymphocytic leukaemia; p53; flow cytometry; prognostic factors;

D O I：

10.1111/j.1365-2141.2004.05223.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

In chronic lymphocytic leukaemia (CLL) cells, functional impairment of the p53 pathway is detectable by Western blotting as impaired up-regulation of p21 (a transcriptional target of p53) in response to ionizing radiation (IR). The type A defect is characterized by baseline p53 overexpression and is associated with TP53 mutation. The type B defect is characterized by impaired IR-induced p53 up-regulation and is associated with inactivation of the ataxia telangiectasia-mutated gene (ATM). Both abnormalities are strongly associated with adverse clinical outcome. In the present study, flow cytometry was found to be an effective alternative to Western blotting in the detection of p53 dysfunction in CLL.

引用

页码：425 / 428

页数：4

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