Role of putative loops 2 and 3 in imipenem passage through the specific porin OprD of Pseudomonas aeruginosa

被引:45
作者
Ochs, MM [1 ]
Bains, M [1 ]
Hancock, REW [1 ]
机构
[1] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, Canada
关键词
D O I
10.1128/AAC.44.7.1983-1985.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Mutant proteins with eight amino acid deletions in putative surface loops 2 and 3 of the imipenem-specific porin OprD of Pseudomonas aeruginosa failed to reconstitute imipenem susceptibility in an oprD-deficient background. The loop 3 deletion prevented the ability of imipenem to inhibit KCl conductance through the OprD channel, as previously shown for a loop 2 deletion. This suggests that both loops 2 and 3 have a role in imipenem binding to the OprD channel.
引用
收藏
页码:1983 / 1985
页数:3
相关论文
共 13 条
[1]  
[Anonymous], 1991, ANTIBIOTICS LAB MED
[2]   THE STRUCTURE OF OMPF PORIN IN A TETRAGONAL CRYSTAL FORM [J].
COWAN, SW ;
GARAVITO, RM ;
JANSONIUS, JN ;
JENKINS, JA ;
KARLSSON, R ;
KONIG, N ;
PAI, EF ;
PAUPTIT, RA ;
RIZKALLAH, PJ ;
ROSENBUSCH, JP ;
RUMMEL, G ;
SCHIRMER, T .
STRUCTURE, 1995, 3 (10) :1041-1050
[3]   CRYSTAL-STRUCTURES EXPLAIN FUNCTIONAL-PROPERTIES OF 2 ESCHERICHIA-COLI PORINS [J].
COWAN, SW ;
SCHIRMER, T ;
RUMMEL, G ;
STEIERT, M ;
GHOSH, R ;
PAUPTIT, RA ;
JANSONIUS, JN ;
ROSENBUSCH, JP .
NATURE, 1992, 358 (6389) :727-733
[4]   NFXC-TYPE QUINOLONE RESISTANCE IN A CLINICAL ISOLATE OF PSEUDOMONAS-AERUGINOSA [J].
FUKUDA, H ;
HOSAKA, M ;
IYOBE, S ;
GOTOH, N ;
NISHINO, T ;
HIRAI, K .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (03) :790-792
[5]   The role of specific surface loop regions in determining the function of the imipenem-specific pore protein OprD of Pseudomonas aeruginosa [J].
Huang, HJ ;
Hancock, REW .
JOURNAL OF BACTERIOLOGY, 1996, 178 (11) :3085-3090
[6]   MEMBRANE TOPOLOGY AND SITE-SPECIFIC MUTAGENESIS OF PSEUDOMONAS-AERUGINOSA PORIN OPRD [J].
HUANG, HJ ;
JEANTEUR, D ;
PATTUS, F ;
HANCOCK, REW .
MOLECULAR MICROBIOLOGY, 1995, 16 (05) :931-941
[7]   THE BACTERIAL PORIN SUPERFAMILY - SEQUENCE ALIGNMENT AND STRUCTURE PREDICTION [J].
JEANTEUR, D ;
LAKEY, JH ;
PATTUS, F .
MOLECULAR MICROBIOLOGY, 1991, 5 (09) :2153-2164
[8]   Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa [J].
Kohler, T ;
MicheaHamzehpour, M ;
Henze, U ;
Gotoh, N ;
Curty, LK ;
Pechere, JC .
MOLECULAR MICROBIOLOGY, 1997, 23 (02) :345-354
[9]   EMERGENCE OF RESISTANCE TO IMIPENEM IN PSEUDOMONAS-AERUGINOSA [J].
LYNCH, MJ ;
DRUSANO, GL ;
MOBLEY, HLT .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1987, 31 (12) :1892-1896
[10]   Negative regulation of the Pseudomonas aeruginosa outer membrane porin OprD selective for imipenem and basic amino acids [J].
Ochs, MM ;
McCusker, MP ;
Bains, M ;
Hancock, REW .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (05) :1085-1090