Differential gene expression of the neural cell adhesion molecule (N-CAM) in a panel of multiple myeloma cell lines

被引:3
作者
De Greef, C [1 ]
Van Riet, I [1 ]
Bakkus, MHC [1 ]
Van Camp, B [1 ]
机构
[1] Free Univ Brussels, Dept Haematol Immunol, B-1090 Brussels, Belgium
关键词
multiple myeloma; N-CAM; CD56; cell lines; gene regulation;
D O I
10.1038/sj.leu.2400897
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
A striking feature of myeloma plasma cells concerns their expression of the neural cell adhesion molecule (N-CAM). The regulation of this particular expression is, however, not known. In this study, the N-CAM (CD56) gene regulation was examined in a panel of multiple myeloma (MM) cell lines. In this panel, both N-CAM-positive and -negative cells were analysed, reflecting the in vivo situation where a minority of MM patients have CD56-negative plasma cells at diagnosis or where in cases of extramedullary involvement CD56 expression decreases. At least two N-CAM mRNAs were found in the cell lines expressing the 140 kDa isoform. With one exception, no N-CAM transcripts could be detected in the N-CAM-negative cell lines. No structural differences could be found in the genomic organization of the N-CAM gene, or in the regulatory promoter region when CD56-positive and -negative cell lines were compared. In transfection studies, however, transcriptional activity of the N-CAM promoter was observed in N-CAM-negative cells, leading us to conclude that the up-regulation of N-CAM in MM cannot be explained by a simple transcriptional gene activation.
引用
收藏
页码:86 / 93
页数:8
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