Three new nuclear genes, sigD, sigE and sigF, encoding putative plastid RNA polymerase σ factors in Arabidopsis thaliana

被引：66

作者：

Fujiwara, M ^{[1
]}

Nagashima, A ^{[1
]}

Kanamaru, K ^{[1
]}

Tanaka, K ^{[1
]}

Takahashi, H ^{[1
]}

机构：

[1] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan

来源：

FEBS LETTERS | 2000年 / 481卷 / 01期

关键词：

sigma factor; multigene family; chloroplast RNA polymerase; transit peptide;

D O I：

10.1016/S0014-5793(00)01965-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three new nuclear genes (sigD, SigE and sigF) of Arabidopsis thaliana, encoding putative plastid RNA polymerase a factors, were identified and analyzed. Phylogenetic analysis revealed that higher plant a factors fell into at least four distinct subgroups within a diverse protein family. In addition, Arabidopsis sig genes contained conserved chromosomal intron sites, indicating that these genes arose ba DNA duplication events during plant evolution. Transcript analyses revealed two alternatively spliced transcripts generated from the sigD region, one of which is predicted to encode a a protein lacking the carboxyterminal regions 3 and 4. Finally, the amino-terminal sequence of the sigF gene product was shown to function as a plastid-targeting signal using green fluorescent protein fusions, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：47 / 52

页数：6

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