Stable isotope dilution method for the determination of guanidinoacetic acid by gas chromatography/mass spectrometry

被引:10
作者
Fingerhut, R [1 ]
机构
[1] Univ Hamburg, Dept Pediat, Hamburg, Germany
关键词
D O I
10.1002/RCM.966
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
For more than 30 years, guanidinoacetic acid (GAA), together with other guanidino compounds, has been proposed as an important marker for renal failure, in kidney transplantation, and for renal metabolism, especially for the metabolic activity of the renal proximal tubules. Since the discovery of the first patient with guanidinoacetic acid methyltransferase deficiency in 1994 by Stockler et al. (Pediatr. Res. 1994; 36: 409), GAA has become of great interest for all laboratories involved in the diagnosis of metabolic diseases. In the literature there are several methods described for the determination of GAA, ranging from ion-exchange chromatography with post-column derivatisation, enzymatic methods, gas chromatography/mass spectrometry (GC/MS), to liquid chromatography/ atmospheric pressure chemical ionisation mass spectrometry (LC/APCI-MS). Here a stable isotope dilution method for quantitative and accurate determination of GAA in urine, plasma, and cerebrospinal fluid is described. GAA is converted to the bis(trifluoromethyl)pyrimidine di(tert-butyldimethylsilyl) derivative by stepwise derivatisation with hexafluoroacetylacetone and N-(tertbutyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA). Analysis can be performed using a standard benchtop GUMS system. For quantitative GAA determination with 1,2-C-13-GAA as internal standard, selected ion monitoring is performed using m/z 460/462, with m/z 432/433 and 375/376 as qualifiers. Copyright (C) 2003 John Wiley Sons, Ltd.
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页码:717 / 722
页数:6
相关论文
共 37 条
[1]  
ALSEVER R N, 1970, Endocrinology, V86, P332
[2]   SIMPLE MONOSUBSTITUTED GUANIDINES OF MAMMALIAN BRAIN [J].
BLASS, JP .
BIOCHEMICAL JOURNAL, 1960, 77 :484-489
[3]   SEPARATION AND ESTIMATION OF CERTAIN GUANIDINO COMPOUNDS - APPLICATION TO HUMAN URINE [J].
BONAS, JE ;
COHEN, BD ;
NATELSON, S .
MICROCHEMICAL JOURNAL, 1963, 7 (01) :63-&
[4]  
CANTONI GL, 1954, J BIOL CHEM, V209, P647
[5]  
COHEN BD, 1985, GUANIDINES, P265
[6]   GUANIDINOACETATE METHYLTRANSFERASE ACTIVITY IN TISSUES AND CULTURED-CELLS [J].
DALY, MM .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1985, 236 (02) :576-584
[7]   GAS-LIQUID-CHROMATOGRAPHIC ELECTRON-CAPTURE DETERMINATION OF SOME MONOSUBSTITUTED GUANIDO-CONTAINING DRUGS [J].
ERDTMANSKY, P ;
GOEHL, TJ .
ANALYTICAL CHEMISTRY, 1975, 47 (04) :750-752
[8]   RECOMBINANT RAT-LIVER GUANIDINOACETATE METHYLTRANSFERASE - REACTIVITY AND FUNCTION OF SULFHYDRYL-GROUPS [J].
FUJIOKA, M ;
KONISHI, K ;
TAKATA, Y .
BIOCHEMISTRY, 1988, 27 (20) :7658-7664
[9]   Guanidinoacetate methyltransferase deficiency: New clinical features [J].
Ganesan, V ;
Johnson, A ;
Connelly, A ;
Eckhardt, S ;
Surtees, RAH .
PEDIATRIC NEUROLOGY, 1997, 17 (02) :155-157
[10]   DEVELOPMENT OF EPILEPTIC SEIZURES THROUGH BRAIN STIMULATION AT LOW INTENSITY [J].
GODDARD, GV .
NATURE, 1967, 214 (5092) :1020-&