The Aer protein of Escherichia coli forms a homodimer independent of the signaling domain and flavin adenine dinucleotide binding

被引：17

作者：

Ma, QH ^{[1
]}

Roy, F ^{[1
]}

Herrmann, S ^{[1
]}

Taylor, BL ^{[1
]}

Johnson, MS ^{[1
]}

机构：

[1] Loma Linda Univ, Div Microbiol & Mol Genet, Loma Linda, CA 92350 USA

来源：

JOURNAL OF BACTERIOLOGY | 2004年 / 186卷 / 21期

关键词：

D O I：

10.1128/JB.186.21.7456-7459.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In vivo cross-linking between native cysteines in the Aer receptor of Escherichia coli showed dimer formation at the membrane anchor and in the putative HAMP domain. Dimers also formed in mutants that did not bind flavin adenine dinucleotide and in truncated peptides without a signaling domain and part of the HAMP domain.

引用

页码：7456 / 7459

页数：4

共 21 条

[1] Collaborative signaling by mixed chemoreceptor teams in Escherichia coli [J].

Ames, P ;

Studdert, CA ;

Reiser, RH ;

Parkinson, JS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (10) :7060-7065

[2] Domain organization and flavin adenine dinucleotide-binding determinants in the aerotaxis signal transducer Aer of Escherichia coli [J].

Bibikov, SI ;

Barnes, LA ;

Gitin, Y ;

Parkinson, JS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (11) :5830-5835

[3] A signal transducer for aerotaxis in Escherichia coli [J].

Bibikov, SI ;

Biran, R ;

Rudd, KE ;

Parkinson, JS .

JOURNAL OF BACTERIOLOGY, 1997, 179 (12) :4075-4079

[4] DEFINITION OF A MINIMAL DOMAIN OF THE DIOXIN RECEPTOR THAT IS ASSOCIATED WITH HSP90 AND MAINTAINS WILD-TYPE LIGAND-BINDING AFFINITY AND SPECIFICITY [J].

COUMAILLEAU, P ;

POELLINGER, L ;

GUSTAFSSON, JA ;

WHITELAW, ML .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (42) :25291-25300

[5] Structure of a flavin-binding plant photoreceptor domain: Insights into light-mediated signal transduction [J].

Crosson, S ;

Moffat, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (06) :2995-3000

[6] PAS domain of the Aer redox sensor requires C-terminal residues for native-fold formation and flavin adenine dinucleotide binding [J].

Herrmann, S ;

Ma, QH ;

Johnson, MS ;

Repik, AV ;

Taylor, BL .

JOURNAL OF BACTERIOLOGY, 2004, 186 (20) :6782-6791

[7] Global conformational changes upon receptor stimulation in photoactive yellow protein [J].

Hoff, WD ;

Xie, A ;

Van Stokkum, IHM ;

Tang, XJ ;

Gural, J ;

Kroon, AR ;

Hellingwerf, KJ .

BIOCHEMISTRY, 1999, 38 (03) :1009-1017

[8] Detecting the conformational change of transmembrane signaling in a bacterial chemoreceptor by measuring effects on disulfide cross-linking in vivo [J].

Hughson, AG ;

Hazelbauer, GL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (21) :11546-11551

[9] Dynamic and clustering model of bacterial chemotaxis receptors: Structural basis for signaling and high sensitivity [J].

Kim, SH ;

Wang, WR ;

Kim, KK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (18) :11611-11615

[10] CATALYTIC OXIDATION OF SULFHYDRYL GROUPS BY O-PHENANTHROLINE COPPER COMPLEX [J].

KOBASHI, K .

BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 158 (02) :239-&

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