Overexpression of archaeal proteins in Escherichia coli

被引：137

作者：

Kim, R

Sandler, SJ

Goldman, S

Yokota, H

Clark, AJ

Kim, SH ^{[1
]}

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Dept Biol Struct, Phys Biosci Div, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

来源：

BIOTECHNOLOGY LETTERS | 1998年 / 20卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1023/A:1005305330517

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Six archaeal proteins containing a high number of Escherichia coli rare codons in their genes were not well expressed in E. coli. These genes showed a five to twenty-fold increase in production when expressed in the presence of a plasmid harboring and expressing the argU and ileX genes encoding rare tRNAs(AGA/AGG)(arg) and tRNA(AUA)(ile).

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收藏

页码：207 / 210

页数：4

相关论文

共 12 条

[1] HIGH-LEVEL EXPRESSION OF RECOMBINANT GENES IN ESCHERICHIA-COLI IS DEPENDENT ON THE AVAILABILITY OF THE DNAY GENE-PRODUCT [J].

BRINKMANN, U ;

MATTES, RE ;

BUCKEL, P .

GENE, 1989, 85 (01) :109-114

[2] High-level misincorporation of lysine for arginine at AGA codons in a fusion protein expressed in Escherichia coli [J].

Calderone, TL ;

Stevens, RD ;

Oas, TG .

JOURNAL OF MOLECULAR BIOLOGY, 1996, 262 (04) :407-412

[3] CONSTRUCTION AND CHARACTERIZATION OF AMPLIFIABLE MULTICOPY DNA CLONING VEHICLES DERIVED FROM P15A CRYPTIC MINIPLASMID [J].

CHANG, ACY ;

COHEN, SN .

JOURNAL OF BACTERIOLOGY, 1978, 134 (03) :1141-1156

[4]

DEBOER HA, 1986, MAXIMIZING GENE EXPR, P225

[5] EFFECTS OF A MINOR ISOLEUCYL TRANSFER-RNA ON HETEROLOGOUS PROTEIN TRANSLATION IN ESCHERICHIA-COLI [J].

DELTITO, BJD ;

WARD, JM ;

HODGSON, J ;

GERSHATER, CJL ;

EDWARDS, H ;

WYSOCKI, LA ;

WATSON, FA ;

SATHE, G ;

KANE, JF .

JOURNAL OF BACTERIOLOGY, 1995, 177 (24) :7086-7091

[6] CODON REPLACEMENT IN THE PGK1 GENE OF SACCHAROMYCES-CEREVISIAE - EXPERIMENTAL APPROACH TO STUDY THE ROLE OF BIASED CODON USAGE IN GENE-EXPRESSION [J].

HOEKEMA, A ;

KASTELEIN, RA ;

VASSER, M ;

DEBOER, HA .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2914-2924

[7] CORRELATION BETWEEN THE ABUNDANCE OF ESCHERICHIA-COLI TRANSFER-RNAS AND THE OCCURRENCE OF THE RESPECTIVE CODONS IN ITS PROTEIN GENES [J].

IKEMURA, T .

JOURNAL OF MOLECULAR BIOLOGY, 1981, 146 (01) :1-21

[8] RECOR SUPPRESSION OF RECF MUTANT PHENOTYPES IN ESCHERICHIA-COLI K-12 [J].

SANDLER, SJ ;

CLARK, AJ .

JOURNAL OF BACTERIOLOGY, 1994, 176 (12) :3661-3672

[9] MISTRANSLATION IN IGF-1 DURING OVER-EXPRESSION OF THE PROTEIN IN ESCHERICHIA-COLI USING A SYNTHETIC GENE CONTAINING LOW-FREQUENCY CODONS [J].

SEETHARAM, R ;

HEEREN, RA ;

WONG, EY ;

BRAFORD, SR ;

KLEIN, BK ;

AYKENT, S ;

KOTTS, CE ;

MATHIS, KJ ;

BISHOP, BF ;

JENNINGS, MJ ;

SMITH, CE ;

SIEGEL, NR .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 155 (01) :518-523

[10] TRANSLATION OF THE SEQUENCE AGG-AGG YIELDS 50-PERCENT RIBOSOMAL FRAMESHIFT [J].

SPANJAARD, RA ;

VANDUIN, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (21) :7967-7971