Hyperpolarization of Human Mesenchymal Stem Cells in Response to Magnetic Force

被引：15

作者：

Kirkham, Glen R. ^{[1
]}

Elliot, Kerry J. ^{[2
]}

Keramane, Ahmed ^{[1
]}

Salter, Donald M. ^{[2
]}

Dobson, Jon P. ^{[1
]}

El Haj, Alicia J. ^{[1
]}

Cartmell, Sarah Harriet ^{[1
]}

机构：

[1] Univ Keele, Inst Sci & Technol Med, Stoke On Trent ST4 7QB, Staffs, England

[2] Univ Edinburgh, Queens Med Res Inst, MRC UoE Ctr Inflammat Res, Edinburgh EH16 4TJ, Midlothian, Scotland

来源：

IEEE TRANSACTIONS ON NANOBIOSCIENCE | 2010年 / 9卷 / 01期

关键词：

BK channels; calcium channel; electrophysiology; magnetic particles; mechanotransduction; mesenchymal stem cells (MSCs); INTRACELLULAR CALCIUM TRANSIENTS; MARROW STROMAL CELLS; SMOOTH-MUSCLE-CELLS; RAT BONE-MARROW; IN-VITRO; VENTRICULAR MYOCYTES; ENDOTHELIAL-CELLS; HUMAN OSTEOBLASTS; ACTIN-FILAMENTS; ION CHANNELS;

D O I：

10.1109/TNB.2010.2042065

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Magnetic particle tagging techniques are currently being applied to tissue engineering applications such as controlled differentiation of mesenchymal stem cells (MSC). In order to define key mechanotransducers underpinning these applications, the electrophysiological responses of human MSCs (hMSC) have been investigated. Ferromagnetic microparticles were coated with L-arginyl-glycyl-L-aspartic acid in order to target the application of dynamic force (6 pN) directly to cell surface integrins. Human MSCs demonstrated cell membrane hyperpolarization responses after the application of force, mediated by BK channels and intracellular calcium release.

引用

页码：71 / 74

页数：4

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