Innate immune activation of swine intestinal epithelial cell lines (IPEC-J2 and IPI-2I) in response to LPS from Salmonella typhimurium

被引：84

作者：

Arce, C. ^{[1
]}

Ramirez-Boo, M. ^{[1
]}

Lucena, C. ^{[1
]}

Garrido, J. J. ^{[1
]}

机构：

[1] Univ Cordoba, Fac Vet, Dept Genet, Unidad Genom & Mejora Anim, E-14071 Cordoba, Spain

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2010年 / 33卷 / 02期

关键词：

Porcine intestinal epithelial cell lines; LPS; TLR; Cytokines; Chemokines; ENTERICA SEROVAR TYPHIMURIUM; TOLL-LIKE RECEPTOR-4; CHEMOATTRACTANT PROTEIN-1 PRODUCTION; INFLAMMATORY-BOWEL-DISEASE; GENE-EXPRESSION; CUTTING EDGE; PROINFLAMMATORY CYTOKINES; DIFFERENTIAL REGULATION; RAPID EXPRESSION; DOWN-REGULATION;

D O I：

10.1016/j.cimid.2008.08.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The innate immune system has the basic function of identifying and eradicating microbial invaders and alerting the adaptative immune system to their presence. In this study, the porcine intestinal innate immune response was evaluated by analysing the expression of TLRs, cytokines and chemokines in two porcine epithelial cell lines from different regions: IPEC-J2 (jejunum) and IPI-2I (ileum). Both cells lines were stimulated with 1 mu g of LIPS from Salmonella typhimurium. RNA was collected at 30 min, 1, 2, 3 and 4 h after treatment. Expression of TLR-1, -2, -3, -4, -6, -8, -9, -10, TNF-alpha, IL-1 beta, -8 and MCP-1 was quantified relative to the quantity of Cyclophilin-A mRNA using real-time quantitative PCR (RTQ-PCR). The results obtained show up differences ill the gene expression between both cell lines IPEC-J2 and IPI-2I as response to LPS from S. typhimurium during the activation time, which may suggest an in vivo variability in the innate immune response against pathogens in different regions of the host's gut. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：161 / 174

页数：14

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