Effect of growth hormone releasing hormone (GHRH) and vasoactive intestinal peptide (VIP) on in vitro bovine oocyte maturation

被引：10

作者：

Beker, ARCL ^{[1
]}

Izadyar, F ^{[1
]}

Colenbrander, B ^{[1
]}

Bevers, MM ^{[1
]}

机构：

[1] Univ Utrecht, Fac Vet Med, Dept Farm Anim Hlth, Utrecht, Netherlands

来源：

THERIOGENOLOGY | 2000年 / 53卷 / 09期

关键词：

IVM; GHRH; VIP; nuclear maturation; cortical granules; cumulus expansion;

D O I：

10.1016/S0093-691X(00)00313-7

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this study was to investigate the effects of growth hormone releasing hormone (GHRH) and the structural-related peptide vasoactive intestinal peptide (VIP) on nuclear maturation, cortical granule distribution and cumulus expansion of bovine oocytes. Bovine cumulus oocyte complexes (COCs) were cultured in M199 without FCS and gonadotropins and in the presence of either 100 ng/mL bovine GHRH or 100 ng/mL porcine VTP. The COCs were incubated at 39 degrees C in a humidified atmosphere with 5% CO2 in air, and the nuclear stage was assessed after 16 or 24 h of incubation using DAPI staining. Cortical granule distribution was assessed after 24 h of incubation using FITC-PNA staining. To assess the effects of GHRH and VIP on cumulus expansion, COCs were incubated for 24 h under the conditions described above. In addition, 0.05 IU/mL recombinant human FSH was added to GHRH and VIP groups. Cultures without GHRH/VIP/FSH or with only FSH served as negative and positive controls, respectively.

引用

页码：1771 / 1782

页数：12

共 64 条

[1] GROWTH HORMONE-RELEASING FACTOR STIMULATES MEIOTIC MATURATION IN FOLLICLE-ENCLOSED AND CUMULUS-ENCLOSED RAT OOCYTE [J].

APA, R ;

LANZONE, A ;

MICELI, F ;

MASTRANDREA, M ;

MACCHIONE, E ;

CARUSO, A ;

MANCUSO, S ;

CANIPARI, R .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1995, 112 (02) :195-201

[2] GROWTH-HORMONE INDUCES IN-VITRO MATURATION OF FOLLICLE-ENCLOSED AND CUMULUS-ENCLOSED RAT OOCYTES [J].

APA, R ;

LANZONE, A ;

MICELI, F ;

MASTRANDREA, M ;

CARUSO, A ;

MANCUSO, S ;

CANIPARI, R .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1994, 106 (1-2) :207-212

[3] Effect of pituitary adenylate cyclase-activating peptide on meiotic maturation in follicle-enclosed, cumulus-enclosed, and denuded rat oocytes [J].

Apa, R ;

Lanzone, A ;

Mastrandrea, M ;

Miceli, F ;

Macchione, E ;

Fulghesu, AM ;

Caruso, A ;

Canipari, R .

BIOLOGY OF REPRODUCTION, 1997, 57 (05) :1074-1079

[4]

ARIMURA A, 1992, REGUL PEPTIDES, V37, P287

[5] GONADOTROPIN-INDUCED EXPRESSION OF RECEPTORS FOR GROWTH-HORMONE RELEASING-FACTOR IN CULTURED GRANULOSA-CELLS [J].

BAGNATO, A ;

MORETTI, C ;

FRAJESE, G ;

CATT, KJ .

ENDOCRINOLOGY, 1991, 128 (06) :2889-2894

[6] EXPRESSION OF THE GROWTH HORMONE-RELEASING HORMONE GENE AND ITS PEPTIDE PRODUCT IN THE RAT OVARY [J].

BAGNATO, A ;

MORETTI, C ;

OHNISHI, J ;

FRAJESE, G ;

CATT, KJ .

ENDOCRINOLOGY, 1992, 130 (03) :1097-1102

[7] THE GLUCAGON SUPERFAMILY - PRECURSOR STRUCTURE AND GENE ORGANIZATION [J].

BELL, GI .

PEPTIDES, 1986, 7 :27-36

[8] Regulation and modulation of oocyte maturation in the bovine [J].

Bevers, MM ;

Dieleman, SJ ;

vandenHurk, R ;

Izadyar, F .

THERIOGENOLOGY, 1997, 47 (01) :13-22

[9] INTERACTION OF OVINE PITUITARY ADENYLATE CYCLASE-ACTIVATING PEPTIDE (PACAP-38) WITH RAT LUNG MEMBRANES [J].

BITAR, KG ;

COY, DH .

PEPTIDES, 1993, 14 (03) :621-627

[10] EFFECTOR MECHANISMS OF PEPTIDES OF THE VIP FAMILY [J].

CHRISTOPHE, J ;

SVOBODA, M ;

LAMBERT, M ;

WAELBROECK, M ;

WINAND, J ;

DEHAYE, JP ;

VANDERMEERSPIRET, MC ;

VANDERMEERS, A ;

ROBBERECHT, P .

PEPTIDES, 1986, 7 :101-107

← 1 2 3 4 5 6 7 →