DNA fragmentation induced by Fe ions in human cells: shielding influence on spatially correlated damage

被引：14

作者：

Antonelli, F

Belli, M

Campa, A

Chatterjee, A

Dini, V

Esposito, G

Rydberg, B

Simone, G

Tabocchini, MA

机构：

[1] Ist Super Sanita, I-00161 Rome, Italy

[2] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USA

来源：

SPACE LIFE SCIENCES: RADIATION RISK ASSESSMENT AND RADIATION MEASUREMENT IN LOW EARTH ORBIT | 2004年 / 34卷 / 06期

关键词：

radiation risk; DNA fragmentation; Fe ions in human cells; radiation shielding; radiation dose;

D O I：

10.1016/j.asr.2003.11.020

中图分类号：

V [航空、航天];

学科分类号：

08 ; 0825 ;

摘要：

Outside the magnetic field of the Earth, high energy heavy ions constitute a relevant part of the biologically significant dose to astronauts during the very long travels through space. The typical pattern of energy deposition in the matter by heavy ions on the microscopic scale is believed to produce spatially correlated damage in the DNA which is critical for radiobiological effects. We have investigated the influence of a Incite shielding on the initial production of very small DNA fragments in human fibroblasts irradiated with 1 GeV/u iron (Fe) ions. We also used gamma-rays as reference radiation. Our results show: (1) a lower effect per incident ion when the shielding is used; (2) an higher DNA Double Strand Breaks (DSB) induction by Fe ions than by gamma-rays in the size range 1-23 kbp; (3) a non-random DNA DSB induction by Fe ions. (C) 2004 COSPAR. Published by Elsevier Ltd. All rights reserved.

引用

页码：1353 / 1357

页数：5

共 13 条

[1]

[Anonymous], MATTER CRITIQUE

[2]

BADHWAR GD, 1997, CP3360 NASA

[3] DNA fragmentation in V79 cells irradiated with light ions as measured by pulsed-field gel electrophoresis. I. Experimental results [J].