Molecular cloning, expression, and antigenicity of Seto virus belonging to genogroup I Norwalk-like viruses

被引：30

作者：

Kobayashi, S

Sakae, K

Suzuki, Y

Shinozaki, K

Okada, M

Ishiko, H

Kamata, K

Suzuki, K

Natori, K

Miyamura, T

Takeda, N

机构：

[1] Aichi Prefectural Inst Publ Hlth, Virol Lab, Kita Ku, Nagoya, Aichi 4628576, Japan

[2] Publ Hlth Lab Chiba Prefecture, Virol Lab, Chuoh Ku, Chiba 2608715, Japan

[3] Mitsubishi Kagaku Bioclin Labs Inc, Infect Dis Test Dev Dept, Itabashi Ku, Tokyo 1748555, Japan

[4] Denka Seiken Co Ltd, Viral Diagnost Prod Dept, Niigata 9591695, Japan

[5] Natl Inst Infect Dis, Dept Biochem & Cell Biol, Sinjuku Ku, Tokyo 1628640, Japan

[6] Natl Inst Infect Dis, Dept Virol 2, Sinjuku Ku, Tokyo 1628640, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 09期

关键词：

D O I：

10.1128/JCM.38.9.3492-3494.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The viral capsid protein of the Seto virus (SeV), a Japanese strain of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like particles using a baculovirus expression system. An antigen detection enzyme-linked immunosorbent assay based on hyperimmune antisera to recombinant SeV was highly specific to homologous SeV-like strains but not heterologous strains in stools, allowing us type-specific detection of NLVs.

引用

页码：3492 / 3494

页数：3

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