Kid, a novel kinesin-like DNA binding protein, is localized to chromosomes and the mitotic spindle

被引:131
作者
Tokai, N
FujimotoNishiyama, A
Toyoshima, Y
Yonemura, S
Tsukita, S
Inoue, J
Yamamoto, T
机构
[1] UNIV TOKYO,INST MED SCI,MINATO KU,TOKYO 108,JAPAN
[2] UNIV TOKYO,DEPT BIOL,MEGURO KU,TOKYO 153,JAPAN
[3] KYOTO UNIV,FAC MED,DEPT BIOCHEM,SAKYO KU,KYOTO 606,JAPAN
关键词
chromosome segregation; kinesin family; kinetochore; mitosis;
D O I
10.1002/j.1460-2075.1996.tb00378.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Microtubule-associated motor proteins are thought to be involved in spindle formation and chromosome movements in mitosis/meiosis. We have molecularly cloned cDNAs for a gene that codes for a novel member of the kinesin family of proteins, Nucleotide sequencing reveals that the predicted gene product is a 73 kDa protein and is related to some extent to the Drosophila nod gene product, which is involved in chromosomal segregation during meiosis, A sequence similar to the microtubule binding motor domain of kinesin is present in the N-terminal half of the protein, and its ability to bind to microtubules is demonstrated, Furthermore, we show that its C-terminal half contains a putative nuclear localization signal similar to that of Jun and is able to bind to DNA, Accordingly, the protein was termed Kid (kinesin-like DNA binding protein), Indirect immunofluorescence studies show that Kid co-localizes with mitotic chromosomes and that it is enriched in the kinetochore at anaphase, Thus, we propose that Kid might play a role(s) in regulating the chromosomal movement along microtubules during mitosis.
引用
收藏
页码:457 / 467
页数:11
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