Isolation by differential display and characterization of a tobacco auxin responsive cDNA Nt-gh3, related to GH3

被引：51

作者：

Roux, C ^{[1
]}

Perrot-Rechenmann, C ^{[1
]}

机构：

[1] CNRS, Inst Sci Vegetales, CNRS, UPR 0040, F-91198 Gif Sur Yvette, France

来源：

FEBS LETTERS | 1997年 / 419卷 / 01期

关键词：

differential display; early auxin-responsive gene; GH3; gene expression; auxin; tobacco;

D O I：

10.1016/S0014-5793(97)01447-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By use of differential display, we have isolated a new early auxin-responsive cDNA in Nicotiana tabacum. Nt-gh3 had 70% identity with the unique GH3 sequence isolated in soybean by Hagen et al, [Planta 162 (1984) 147-153] and is thus the first reported cDNA related to this gene until now. Nt-gh3 mRNA accumulates within a short time after auxin treatment, responds to very low concentrations of NAA (as little as 10(-9) M) and specifically to active auxins. Nt-gh3 mRNA is demonstrated to be one of the most relevant early molecular markers of primary auxin response. (C) 1997 Federation of European Biochemical Societies.

引用

页码：131 / 136

页数：6

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