Migration of enhanced green fluorescent protein expressing bone marrow-derived microglia/macrophage into the mouse brain following permanent focal ischemia
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作者:
Tanaka, R
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机构:Juntendo Univ, Sch Med, Dept Neurol, Bunkyo Ku, Tokyo 1130033, Japan
Tanaka, R
Komine-Kobayashi, M
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机构:Juntendo Univ, Sch Med, Dept Neurol, Bunkyo Ku, Tokyo 1130033, Japan
Komine-Kobayashi, M
Mochizuki, H
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机构:Juntendo Univ, Sch Med, Dept Neurol, Bunkyo Ku, Tokyo 1130033, Japan
Mochizuki, H
Yamada, M
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机构:Juntendo Univ, Sch Med, Dept Neurol, Bunkyo Ku, Tokyo 1130033, Japan
Yamada, M
Furuya, T
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机构:Juntendo Univ, Sch Med, Dept Neurol, Bunkyo Ku, Tokyo 1130033, Japan
Furuya, T
Migita, M
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机构:Juntendo Univ, Sch Med, Dept Neurol, Bunkyo Ku, Tokyo 1130033, Japan
Migita, M
Shimada, T
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机构:Juntendo Univ, Sch Med, Dept Neurol, Bunkyo Ku, Tokyo 1130033, Japan
Shimada, T
Mizuno, Y
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机构:Juntendo Univ, Sch Med, Dept Neurol, Bunkyo Ku, Tokyo 1130033, Japan
Mizuno, Y
Urabe, T
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机构:Juntendo Univ, Sch Med, Dept Neurol, Bunkyo Ku, Tokyo 1130033, Japan
Urabe, T
机构:
[1] Juntendo Univ, Sch Med, Dept Neurol, Bunkyo Ku, Tokyo 1130033, Japan
[2] Nippon Med Coll, Dept Biochem & Mol Biol, Tokyo 1138502, Japan
Brain ischemia induces a marked response of resident microglia and hematopoietic cells including monocytes/macrophages. The present study was designed to assess the distribution of microglia/macrophages in cerebral ischemia using bone marrow chimera mice known to express enhanced green fluorescent protein (EGFP). At 24 h after middle cerebral artery occlusion (MCAO), many round-shaped EGFP-positive cells migrated to the ischemic core and peri-infarct area. At 48-72 h after MCAO, irregular round-or oval-shaped EGFP/ionized calcium-binding adapter molecule 1 (Iba 1)-positive cells increased in the transition zone, while many amoeboid-shaped or large-cell-body EGFP/Iba I-positive cells were increased in number in the innermost area of ischemia. At 7 days after MCAO, many process-bearing ramified shaped EGFP/Iba 1-positive cells were detected in the transition to the peri-infarct area, while phagocytic cells were distributed in the transition to the core area of the infarction. The distribution of these morphologically variable EGFP/Iba 1-positive cells was similar up to 14 days from MCAO. The present study directly showed the migration and distribution of bone marrow-derived monocytes/macrophages and the relationship between resident microglia and infiltrated hematogenous element in ischemic mouse brain. It is important to study the distribution of intrinsic and extrinsic microglia/macrophage in ischemic brain, since such findings may allow the design of appropriate gene-delivery system using exogenous microglia/macrophages to the ischemic brain area. (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.
机构:
UNIV CALIF LOS ANGELES, MED CTR, JONSSON COMPREHENS CANC CTR, LOS ANGELES, CA 90024 USAUNIV CALIF LOS ANGELES, MED CTR, JONSSON COMPREHENS CANC CTR, LOS ANGELES, CA 90024 USA
CHIANG, CS
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MCBRIDE, WH
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UNIV CALIF LOS ANGELES, MED CTR, JONSSON COMPREHENS CANC CTR, LOS ANGELES, CA 90024 USAUNIV CALIF LOS ANGELES, MED CTR, JONSSON COMPREHENS CANC CTR, LOS ANGELES, CA 90024 USA
MCBRIDE, WH
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WITHERS, HR
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机构:
UNIV CALIF LOS ANGELES, MED CTR, JONSSON COMPREHENS CANC CTR, LOS ANGELES, CA 90024 USAUNIV CALIF LOS ANGELES, MED CTR, JONSSON COMPREHENS CANC CTR, LOS ANGELES, CA 90024 USA
机构:
UNIV CALIF LOS ANGELES, MED CTR, JONSSON COMPREHENS CANC CTR, LOS ANGELES, CA 90024 USAUNIV CALIF LOS ANGELES, MED CTR, JONSSON COMPREHENS CANC CTR, LOS ANGELES, CA 90024 USA
CHIANG, CS
;
MCBRIDE, WH
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h-index: 0
机构:
UNIV CALIF LOS ANGELES, MED CTR, JONSSON COMPREHENS CANC CTR, LOS ANGELES, CA 90024 USAUNIV CALIF LOS ANGELES, MED CTR, JONSSON COMPREHENS CANC CTR, LOS ANGELES, CA 90024 USA
MCBRIDE, WH
;
WITHERS, HR
论文数: 0引用数: 0
h-index: 0
机构:
UNIV CALIF LOS ANGELES, MED CTR, JONSSON COMPREHENS CANC CTR, LOS ANGELES, CA 90024 USAUNIV CALIF LOS ANGELES, MED CTR, JONSSON COMPREHENS CANC CTR, LOS ANGELES, CA 90024 USA