Na+-dependent fructose transport via rNaGLT1 in rat kidney

We found a system of Na+-dependent uptake of fructose by rat renal brush-border vesciles. It consisted of two saturablen components and was thought to involve at least two transporters. rNaGLT1, a novel glucose transporter in rat kidney, showed fructose uptake as well as alpha-methyl-(D)-glucopyranoside uptake by transfected HEK293 cells. The features of the lower affinity type of fructose transporter in the brush-border membranes, such as affinity and substrate recognition, were very comparable with those of rNaGLT1-transfected HEK293 cells. These results inducated that rNaGLT1 is a primary fructose transporter in rat renal brush-border membranes. (C) 2003 Published by Elsevier Science B.V. on behalf ofthe Federation of European Biochemical Societies.