λ-red genetic engineering in Salmonella enterica serovar typhimurium

被引：96

作者：

Karlinsey, Joyce E. ^{[1
]}

机构：

[1] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA

来源：

ADVANCED BACTERIAL GENETICS: USE OF TRANSPOSONS AND PHAGE FOR GENOMIC ENGIEERING | 2007年 / 421卷

关键词：

D O I：

10.1016/S0076-6879(06)21016-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The use of the recombination system from bacteriophage lambda, lambda-Red, allows for PCR-generated fragments to be targeted to specific chromosomal locations in sequenced genomes. A minimal region of homology of 30 to 50 bases flanking the fragment to be inserted is all that is required for targeted mutagenesis. Procedures for creating specific insertions, deletions, and site-directed changes are described.

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页码：199 / 209

页数：11

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